LightSheet Microscopy

Light sheet microscopy addresses two significant challenges in modern microscopy: it allows for extended imaging of biological samples under physiologically relevant conditions and enables the imaging of larger samples within a more reasonable time frame compared to conventional techniques.

This method involves illuminating the sample with a sheet of light oriented perpendicular to the imaging axis, focusing only on the plane of interest. Consequently, out-of-focus structures receive less light exposure, reducing the likelihood of phototoxic effects on the sample.

Light sheet microscopy effectively combines the advantages of both worlds. It enhances signal-to-noise ratios compared to widefield illumination and improves acquisition speed by utilizing a camera instead of a point scanner, as seen in confocal microscopy.